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目的检测PRDM14在肺癌细胞系中的表达及分布情况。方法采用RT-PCR技术和免疫荧光方法分别检测人支气管上皮细胞系(HBE)和6个肺癌细胞系中PRDM14mRNA和蛋白的表达。结果 RT-PCR结果显示PRDM14与GAPDH电泳条带的平均光密度比值在肺癌细胞系中显著高于HBE细胞系(P<0.001);免疫荧光结果显示PRDM14蛋白表达的平均荧光强度在肺癌细胞系中高于HBE细胞系(P<0.05),PRDM14蛋白荧光信号主要定位于细胞浆。结论 PRDM14在HBE细胞系低表达,在肺癌细胞系高表达,主要分布于细胞浆,PRDM14可能在肺癌的发生发展中发挥作用。
Objective To detect the expression and distribution of PRDM14 in lung cancer cell lines. Methods The expression of PRDM14 mRNA and protein in human bronchial epithelial cell line (HBE) and 6 lung cancer cell lines were detected by RT-PCR and immunofluorescence. Results The RT-PCR results showed that the average optical density ratio of PRDM14 and GAPDH bands in lung cancer cell lines was significantly higher than that in HBE cell lines (P <0.001). Immunofluorescence results showed that the average fluorescence intensity of PRDM14 protein expression in lung cancer cell lines was high In the HBE cell line (P <0.05), the fluorescence signal of PRDM14 protein mainly localized in the cytoplasm. Conclusions PRDM14 is low expressed in HBE cell lines and highly expressed in lung cancer cell lines, mainly in the cytoplasm. PRDM14 may play a role in the development of lung cancer.