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对大豆花叶病毒(SMA),花生斑驳病毒(PMV)和豇豆蚜传花叶病毒(CAMV)3种Potyvirus病毒进行了免疫电镜(ISEM)的测定。ISEM法同样证实了这3种病毒在血清学关系上的不同。3种病毒抗血清对同源抗原都具有“捕获”大量病毒粒体的能力,“捕获”数量是异源关系或正常血清的20～50倍.ISEM方法的灵敏度和专化性受到包被抗血清的稀释度和在抗原上处理时间等因素的影响.“捕获”病毒粒体最适的抗血清稀释度是10~(-3)或10~(-4);抗原处理时间,在一定范围内随处理时间的延长而增加“捕获”病毒粒体的数量,处理时间超过10—20小时即不再增加“捕获”粒体数量.同源抗体抗—抗原反应对病毒粒体上有“装饰”作用,而异源关系没有“装饰”作用。“装饰”的免疫电镜法是鉴别同源病毒的重要标准。
Immunoelectron microscopy (ISEM) was used to detect the three Potyvirus viruses including soma mosaic virus (SMA), peanut mottle virus (PMV) and cowpea aphid-transmitted mosaic virus (CAMV) ISEM also confirmed the serological differences between the three viruses. The three virus antisera had the ability to “capture” a large number of virions for the cognate antigen, with 20 to 50 times more “capture” than the heterologous or normal sera.The sensitivity and specificity of the ISEM method were affected by the coating resistance Serum dilution and antigen processing time and other factors. “Capture” virus mitochondria optimum antisera dilution is 10 ~ (-3) or 10 ~ (-4); antigen processing time, within a certain range Increasing the number of “captured” virions within the timeframe of treatment increases the number of “trapped” particles beyond 10-20 hours Homologous Antibodies Anti-Antigen Response The number of “ ”Role, while there is no“ decorative ”effect of the heterologous relationship. “Decorative” immunoelectron microscopy is an important criterion for identifying homologous viruses.