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用胎鼠的限定骨骼肌提取液（ＭＥ，１０～５０ｋＤａ）作用于无血清培养的胚胎大鼠脊髓腰段腹角细胞后，用四唑盐（ＭＴＴ）微量自动比色定量法和ＬＤＨ活性测定法检测了骨骼肌提取液对大鼠脊髓运动神经元生长存活的生物活性，结果显示：细胞培养４ｄｉｖ后，ＭＴＴ微量比色的ＯＤ值随加入骨骼肌提取液浓度的增加而升高，含骨骼肌提取液２００和４００μｇ／ｍｌ蛋白的实验孔与单纯无血清的对照孔比较有极显著性差异（Ｐ＜０．００１），说明２００μｇ／ｍｌ以上浓度的骨骼肌提取液对脊髓腹角神经元的生长有明显的促进作用。细胞培养１４ｄｉｖ后，ＬＤＨ活性测定ＯＤ值的线性回归斜率亦随加入骨骼肌提取液浓度的增加而升高，说明存活细胞数的增加。实验说明培养神经细胞的ＭＴＴ和ＬＤＨ的检测方法有可能成为评估运动神经营养因子活性的指标。
The fetal skeletal muscle extract (ME, 10 ~ 50kDa) was used to effect the determination of LDH activity in the spinal cord of the lumbar spinal cord of serum-free embryos in rats. Method was used to detect the biological activity of skeletal muscle extract on the growth and survival of spinal motor neurons in rats. The results showed that the OD value of MTT trace colorimetric increased with the increase of the concentration of skeletal muscle extract, There was a significant difference (P <0.001) between the experimental wells of 200 and 400 μg / ml protein extracts of muscle and serum-free control wells, indicating that skeletal muscle extracts at concentrations above 200 μg / The growth of a significant role in promoting. The linear regression slope of the OD value of the LDH activity assay increased with the increase of the concentration of skeletal muscle extract after the cell culture of 14div, indicating an increase in the number of surviving cells. Experiments show that the detection of MTT and LDH cultured neurons may become an indicator of the activity of motor neurotrophic factor.