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本文介绍了用以显示碱性磷酸酶活性的铅沉淀法的碱性磷酸酶-抗碱性磷酸酶(APA-AP)免疫组织化学技术,以及使用计算机图像处理进行显微光密度测量确定的该技术理想反应条件.其条件是:孵育液硝酸铅浓度为3mmol/L,底物浓度1.96～31.36mmol/L,pH值7～9;APAAP浓度1:50;一级抗体、第一和第二桥抗体的浓度分别为1:1 000、1:100和1:200。讨论了APAAP-铅沉淀技术的某些优点。
This article presents the alkaline phosphatase-anti-alkaline phosphatase (APA-AP) immunohistochemistry technique of lead precipitation to show alkaline phosphatase activity, as well as the use of computerized image processing for the determination of micro-densitometry Technical conditions of the reaction conditions: the incubation solution of lead nitrate concentration of 3mmol / L, the substrate concentration of 1.96 ~ 31.36mmol / L, pH 7 ~ 9; APAAP concentration of 1:50; primary antibody, the first and second The concentration of bridging antibody was 1: 1 000, 1: 100 and 1: 200 respectively. Some of the advantages of the APAAP-lead precipitation technique are discussed.