Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for ba

• 作 者: Barbara J. CHANG
• 机 构: Department of Microbiology and Immunology School of Biomedical Biomolecular and Chemical Sciences Un
• 出 处: Journal of Zhejiang University(Science B:An International Bi
• 发表日期: 2008年04期
• 关键词: suspension probes covalently utility correctly validate beads universal template

The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.

The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound Thirty-eight strains belonging to 15 species were identified correctly on the basis of their corresponding species-specific hybridization profiles. results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.