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引发作为种子播前处理技术,可以提高逆境条件下种子活力。本文探讨了盐胁迫下杂交一代番茄2个品种(‘大红合作909’和‘粉红合作906’)幼苗不同部位对蛭石引发的响应。利用蛭石在15°C黑暗条件下对2个番茄品种的种子引发处理5 d,引发和未引发的种子分别放入浓度为0、100 mmol·L~(-1) NaCl盐溶液的发芽盒内,(25±1)°C发芽10 d后取根、茎、叶进行各项生理指标的测定。研究显示,与未引发种子相比,蛭石引发能显著提高幼苗茎、根干重和鲜重。此外,蛭石引发能显著降低盐胁迫下茎、根中丙二醛含量,提高根、茎脯氨酸含量、抗氧化酶活性(SOD、POD、CAT),积累较多渗透调节物质(游离氨基酸、可溶性蛋白、可溶性糖),提高了叶绿素含量;且发现叶绿素b(Chlb)比叶绿素a(Chla),类胡萝卜素(carotenoid,Car)比叶绿素(a+b)在盐胁迫下稳定。结果表明:蛭石引发能够提高盐胁迫下番茄幼苗不同器官抗氧化酶活性,促进渗透调节物质的合成,降低膜透性和膜脂过氧化水平,保护光合器官及细胞膜结构的稳定性,从而提高番茄幼苗的耐盐能力。
Triggered as seed sowing pretreatment technology, can improve seed vigor under adverse conditions. In this paper, we investigated the responses of two cultivars of hybrid tomato (salt red 909 and pink 906) to the initiation of vermiculite under salt stress. Seed germination of two tomato cultivars was induced by vermiculite at 15 ° C for 5 days. The primed and untreated seeds were placed in germination boxes containing 0,100 mmol·L -1 NaCl salt solution Within (25 ± 1) ° C after 10 days of germination, root, stem and leaf were taken for determination of physiological indexes. Studies have shown that vermiculite priming can significantly increase seedling stems, root dry weight and fresh weight compared to non-primed seeds. In addition, vermiculite priming could significantly reduce the content of malondialdehyde (MDA) in stems and roots, increase the content of proline and antioxidant enzymes (SOD, POD, CAT) in roots and stems, and accumulate more osmolytes (free amino acids , Soluble protein and soluble sugar) increased the content of chlorophyll. Chlb was more stable than that of chlorophyll (a + b) than that of chlorophyll a (Chla) and carotenoid (Carbenoid) under salt stress. The results showed that vermiculite priming enhanced the antioxidant enzyme activities of different organs of tomato seedlings under salt stress, promoted the synthesis of osmolytes, decreased the membrane permeability and membrane lipid peroxidation, and protected the photosynthetic organs and the stability of cell membrane structure, thereby increasing Salt tolerance of tomato seedlings.