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目的研究了解壳寡糖的毒性。方法将大鼠分为阴性对照组及低、中、高3个剂量组。连续喂养30d后,采血作血常规及生化指标测定,取出肝、肾、脾、睾丸称重,并对肝、肾、脾、胃肠、睾丸、卵巢作组织病理学检查。结果各剂量组大鼠每周体重及其增重、总进食量、总食物利用率、脏体比与对照组差异无统计学意义。血生化中雄性低剂量组谷丙转氨酶、中剂量组尿素、血糖低于对照组;血常规中雄性高剂量组中性粒细胞、各剂量组单核细胞与对照组差异有统计学意义,但均在本实验室正常值范围内,故认为无生物学意义;其他各剂量组动物各项血生化、血常规指标与对照组差异无统计学意义。对受检脏器作组织病理学检查,未见特异性病变。结论服用壳寡糖30d不产生亚急性毒性,对机体未见不良影响。
Objective To understand the toxicity of chitooligosaccharides. Methods The rats were divided into negative control group and low, medium and high dose groups. After continuous feeding for 30 days, the blood was taken for blood routine and biochemical determination. The liver, kidney, spleen and testis were taken out and weighed for histopathology of liver, kidney, spleen, gastrointestinal tract, testis and ovary. Results There was no significant difference in body weight, weight gain, total food intake, total food utilization rate, and body weight ratio of rats in each dose group compared with the control group. Blood levels of alanine aminotransferase in the low-dose group, urea and blood glucose in the middle-dose group were lower than those in the control group. The levels of neutrophils in the high-dose group and the monocytes in each dose group were significantly different from those in the control group Are within the normal range of the laboratory, so that no biological significance; other various dose groups of animal blood biochemical, blood parameters and the control group no significant difference. On the subject organs for histopathological examination, no specific lesions. Conclusion Chitooligosaccharides did not produce subacute toxicity for 30 days and had no adverse effect on the body.