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目的探讨氟、砷单独及其联合染毒对大鼠在新环境中的探究行为的影响及机制。方法将96只健康初断乳SPF级SD大鼠随机分为对照(蒸馏水)组和NaF(120 mg/L)染毒组、亚砷酸钠(70 mg/L)染毒组及NaF(120 mg/L)+亚砷酸钠(70 mg/L)染毒组,每组24只,雌雄各半。采用饮水方式连续染毒3个月。采用旷场试验测试大鼠在新环境中的探究行为,检测大鼠血清和海马组织匀浆中总抗氧化能力(T-AOC)水平和丙二醛(MDA)、一氧化氮(NO)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和一氧化氮合酶(NOS)活力。结果 NaF+亚砷酸钠染毒组大鼠旷场试验总得分低于对照组、NaF和亚砷酸钠染毒组(P<0.01)。NaF、亚砷酸钠和NaF+亚砷酸钠染毒组中央格停留时间均长于对照组(P<0.01)。与对照组比较,NaF、亚砷酸钠和NaF+亚砷酸钠染毒组大鼠血清T-AOC水平及亚砷酸钠染毒组和NaF+亚砷酸钠染毒组血清SOD、GSH-Px活力降低,MDA含量升高(P<0.05,P<0.01)。与NaF染毒组比较,亚砷酸钠和NaF+亚砷酸钠染毒组大鼠血清中T-AOC水平和GSH-Px活力降低(P<0.05,P<0.01)。与对照组比较,NaF、亚砷酸钠和NaF+亚砷酸钠染毒组大鼠海马组织匀浆中T-AOC水平均降低(P<0.01)。与亚砷酸钠染毒组比较,对照组、NaF染毒组和NaF+亚砷酸钠染毒组大鼠海马组织匀浆中GSH-Px的活力均较高(P<0.01)。与NaF+亚砷酸钠染毒组比较,NaF、亚砷酸钠染毒组和对照组大鼠海马组织匀浆中MDA含量均较低(P<0.01)。与对照组比较,NaF和NaF+亚砷酸钠染毒组大鼠血清NO含量以及NaF、亚砷酸钠和NaF+亚砷酸钠染毒组大鼠血清中NOS活力及海马组织中NO含量、NOS活力均下降(P<0.05,P<0.01)。结论氟、砷及其联合作用可损害大鼠在新环境中的探究行为,其机制可能与海马组织氧化损伤及NO含量和NOS活力降低有关。
Objective To investigate the effects of fluoride and arsenic alone and in combination on the exploring behavior of rats in new environment and its mechanism. Methods Ninety-six SPF SD rats were randomly divided into control (distilled water) group and NaF (120 mg / L), sodium arsenite (70 mg / L) mg / L) + sodium arsenite (70 mg / L), with 24 mice in each group. Continuous drinking water for 3 months. The open field test was used to test the exploring behavior of rats in a new environment. The levels of T-AOC, MDA and NO in serum and hippocampus of rats were measured. And superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and nitric oxide synthase (NOS) activity. Results The total open field test scores of rats in NaF plus sodium arsenite group were lower than those in control group, NaF group and sodium arsenite group (P <0.01). The median residence time of NaF, sodium arsenite and NaF + sodium arsenite groups were longer than that of the control group (P <0.01). Compared with the control group, the levels of serum T-AOC in NaF, sodium arsenite and NaF + sodium arsenite-treated rats and the serum levels of SOD and GSH-Px in sodium arsenite and NaF + Decreased vitality, MDA content increased (P <0.05, P <0.01). Compared with the NaF group, the levels of T-AOC and GSH-Px in the serum of sodium arsenite and NaF + sodium arsenite-treated rats decreased (P <0.05, P <0.01). Compared with the control group, the levels of T-AOC in the hippocampal homogenate of NaF, sodium arsenite and NaF + sodium arsenite exposure groups were decreased (P <0.01). Compared with the sodium arsenite treatment group, the activity of GSH-Px in the hippocampal tissue homogenate of the control group, NaF and NaF + sodium arsenite treatment groups were higher (P <0.01). Compared with NaF + sodium arsenite exposure group, the content of MDA in hippocampal homogenate of NaF, arsenite-treated and control groups was lower (P <0.01). Compared with the control group, the levels of serum NO in NaF and NaF + sodium arsenite-treated rats and the activities of NOS in hippocampus and NOS in NaF, sodium arsenite and NaF + sodium arsenite-treated rats, Vitality decreased (P <0.05, P <0.01). Conclusion Fluorine, arsenic and their combined actions may impair the exploring behavior of rats in new environment. The mechanism may be related to the oxidative damage of hippocampus and the decrease of NO content and NOS activity.