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目的探讨斑贞1号联合氟尿嘧啶(5-FU)对胃癌SGC-7901/ADR细胞的细胞毒作用。方法以SGC-7901/ADR细胞为靶细胞,分别设5-FU组、斑贞1号组、斑贞1号+5-FU组,采用四甲基偶氮唑盐(MTT)法观察各药物组的细胞毒作用,光镜下动态拍照并用流式细胞仪测定各药物组细胞周期的变化。结果斑贞1号联合5-FU半数抑制浓度(IC50)分别由单独用药时的(7.85±0.03)mg/L(、13.00±0.13)mg/L降至(4.75±0.04)mg/L(P<0.01)。对SGC-7901/ADR细胞毒作用斑贞1号+5-FU>斑贞1号>5-FU。光镜下斑贞1号+5-FU组细胞与单独用药组相比,细胞生长明显受到抑制,癌细胞体积变小,胞体全面皱缩。流式细胞仪检测斑贞1号+5-FU组SGC-7901/ADR细胞明显阻滞在G0/G1期,进入S期细胞减少,抑制癌细胞DNA的合成(P<0.05)。结论斑贞1号联合5-FU较单独用药对SGC-7901/ADR细胞具有明显的细胞毒作用。
Objective To investigate the cytotoxic effect of Dachen No.1 combined with 5-fluorouracil (5-FU) on gastric cancer SGC-7901 / ADR cells. Methods SGC-7901 / ADR cells were used as target cells, and 5-FU, Zhen-zhen-1 and Zhen-zhen-1 + 5-FU groups were respectively treated with MTT method. Group of cytotoxicity, dynamic photomicrographs and determination by flow cytometry of each drug group cell cycle changes. Results The median inhibitory concentration (IC50) of Zhenzhen 1 combined with 5-FU decreased from (7.85 ± 0.03) mg / L (13.00 ± 0.13) mg / L to 4.75 ± 0.04 mg / L <0.01). Cytotoxicity on SGC-7901 / ADR FEN Zhen-1 + 5-FU> FANG Zhen-1> 5-FU. Compared with the drug alone group, the cell growth was significantly inhibited in the cells of the Dianzhen 1 + 5-FU group under the light microscope, the volume of the cancer cells became smaller and the cell bodies were fully collapsed. Flow cytometry showed that SGC-7901 / ADR cells were significantly arrested in G0 / G1 phase and decreased in S phase and inhibited DNA synthesis (P <0.05). Conclusion Dachen 1 combined with 5-FU has obvious cytotoxic effect on SGC-7901 / ADR cells.