Reversible Immobilization of Chelating Affinity Surfactants on Reversed Phase Adsorbents for Protein

• 作 者: Omar González-Ortega Robe
• 机 构: ChemicalandEnvironmentalEngineeringDepartment,BioprocessEngineeringDepartment
• 出 处: 美国分析化学(英文)
• 发表日期: 2014年14期
• 关键词: IMAC Brij CHELATING Surfactant RESTRICTED Access Media Alkyl-Bound Silica IMAC B

Alkyl-bound silica was modified using chelating surfactants and the resulting adsorbent was used in immobilized metal affinity chromatography of proteins and peptides. Brij-76, a non-ionic amphiphilic surfactant with an alkyl moiety and an ethylene oxide