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目的:探讨TRAIL联合白藜芦醇对乳腺癌MDA-MB-231细胞株增殖及凋亡的影响。方法:MTT法和软琼脂集落形成实验检测细胞增殖;DAPI染色法及流式细胞术检测细胞凋亡;流式细胞术检测细胞表面DR4、DR5蛋白表达。结果:50 ng/mL TRAIL与50和100μmol/L白藜芦醇联合作用14 d后,MDA-MB-231细胞在软琼脂中集落形成率分别为(2.49±0.08)%和(1.38±0.07)%,与相应浓度TRAIL、白藜芦醇单独应用比较及组间比较,差异有统计学意义,P<0.01。50 ng/mL TRAIL与50和100μmol/L白藜芦醇联合作用后,对MDA-MB-231细胞增殖抑制率为(62.43±2.07)%、(87.32±1.44)%;细胞凋亡率为(53.59±1.44)%、(73.72±1.96)%,与单独应用相应浓度TRAIL、白藜芦醇比较及组间比较,差异有统计学意义,P<0.01。50、100和200μmol/L白藜芦醇作用后,细胞膜表面DR4、DR5的荧光指数分别为1.80±0.07、2.11±0.14、2.59±0.16和2.16±0.08、2.92±0.25、3.90±0.06,与对照组(FI值分别为1.52±0.04和1.61±0.13)比较及组间比较差异有统计学意义,P<0.05。结论:TRAIL与白藜芦醇联合可协同抑制MDA-MB-231细胞增殖并诱导其凋亡,DR4、DR5蛋白表达的上调可能是其协同效应的机制之一。
Objective: To investigate the effect of TRAIL combined with resveratrol on the proliferation and apoptosis of breast cancer cell line MDA-MB-231. Methods: Cell proliferation was detected by MTT assay and soft agar colony formation assay. Apoptosis was detected by DAPI staining and flow cytometry. Flow cytometry was used to detect the expression of DR4 and DR5. RESULTS: After 14 days of 50 ng / mL TRAIL combined with 50 and 100 μmol / L resveratrol, the colony formation rates of MDA-MB-231 cells in soft agar were (2.49 ± 0.08)% and (1.38 ± 0.07) %, Respectively, compared with the corresponding concentration of TRAIL, Resveratrol alone and compared between groups, the difference was statistically significant, P <0.01.50 ng / mL TRAIL combined with 50 and 100μmol / L resveratrol, MDA (62.43 ± 2.07)% and (87.32 ± 1.44)%, respectively. The rate of apoptosis was (53.59 ± 1.44)% and (73.72 ± 1.96)%, respectively. Compared with the single application of the corresponding concentration of TRAIL, (P <0.01). After treated with 100, 200 and 200 μmol / L resveratrol, the fluorescence indices of DR4 and DR5 on the membrane surface were 1.80 ± 0.07 and 2.11 ± 0.14, respectively , 2.59 ± 0.16 and 2.16 ± 0.08,2.92 ± 0.25,3.90 ± 0.06, respectively, compared with the control group (FI values were 1.52 ± 0.04 and 1.61 ± 0.13 respectively), and the differences between the two groups were statistically significant (P <0.05). CONCLUSION: The combination of TRAIL and resveratrol synergistically inhibits the proliferation and induces the apoptosis of MDA-MB-231 cells. The upregulation of DR4 and DR5 may be one of the mechanisms of synergistic effect.