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采用固相微萃取和气质联用技术,对健康棉叶、蚜害棉叶、蚜害棉叶-棉蚜复合物、茉莉酸甲酯处理棉叶以及棉蚜蜜露和棉蚜自身挥发物成分进行了提取和组分分析。共检测出27种挥发性化学物质。健康棉叶、蚜害棉叶、蚜害棉叶-棉蚜复合物、茉莉酸甲酯处理棉叶挥发物中,均含α-蒎烯、β-蒎烯、β-石竹烯、十五烷、十六烷、十七烷、-Cyclohexen-1-ol,1-(1,5-dimethyl-4-hexenyl)-4-methyl-7种组份。蚜害棉叶或蚜害棉叶-棉蚜复合物中检测到α-水芹烯、α-石竹烯、α-法尼烯、2,6,10-三甲基十五烷、(E,Z)-2,6-二甲基-2,4,6-辛三烯、2,6,10,14-四甲基十五烷、蒽、二酚基丙烷8种组份;3-蒈烯、(E)-β-法尼烯只在蚜害棉叶及茉莉酸甲酯处理棉叶中被检测出。在棉蚜及其蜜露中都能检查到壬醛、癸醛、十四烷、十五烷、十六烷、十七烷;而1-甲氧基-4-[(Z)-1-丙烯基]苯、(E)-β-法尼烯、菲只在棉蚜蜜露中被检查到;6,10-二甲基-5,9-十一双烯-2-酮只在棉蚜中被检测到。研究结果为进一步开展棉花、棉蚜挥发物对天敌昆虫的吸引作用研究奠定了基础。
Solid phase microextraction (GC-MS) and gas chromatography-mass spectrometry (GC-MS) were used to detect the volatile components of healthy cotton leaves, aphid cotton leaves, aphid cotton leaf-cotton aphid complex, methyl jasmonate treated cotton leaf, Extraction and component analysis were performed. A total of 27 volatile chemicals were detected. Health cotton, aphid cotton leaves, aphid cotton leaf - cotton aphid complex, methyl jasmonate treatment of cotton volatiles, contain α-pinene, β-pinene, β-caryophyllene, pentadecane , Hexadecane, heptadecane, Cyclohexen-1-ol, 1- (1,5-dimethyl-4-hexenyl) -4-methyl-7 components. Α-phenethyl, α-farnesene, 2,6,10-trimethylpentadecane, (E, N-dimethylaminopyridine) were detected in the cotton aphid- Z) -2,6-dimethyl-2,4,6-octatriene, 2,6,10,14-tetramethylpentadecane, anthracene, and diphenolpropane; Alkene, (E) -β-farnesene was detected only in cotton leaves treated with aphids and methyl jasmonate. Nonanal, decanal, tetradecane, pentadecane, hexadecane, and heptadecane were detected in both cotton aphid and honeydew, whereas 1-methoxy-4 - [(Z) -1- Propenyl] benzene, (E) -β-farnesene, phenanthrene was only detected in cotton aphid nectar; 6,10-dimethyl-5,9-undecanediene-2-one only in cotton Aphids were detected. The results laid the foundation for further studies on the attraction of cotton and cotton aphid to natural enemy insects.