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目的通过研究急性羰基镍中毒大鼠各脏器抗超氧阴离子含量变化,探讨大鼠羰基镍中毒后的毒作用和微观机制。方法SD健康大鼠静态吸入方式染毒,羰基镍染毒剂量分别为20、135、250mg/m3,设染毒对照组氯气剂量为250mg/m3,染毒时间均为30min,并设正常对照组,染毒后第1、第2、第3、第7天取材;采用化学显色法测定抗超氧阴离子含量。结果在染毒第1天时,高剂量染毒组肺脏中抗超氧阴离子为119.70U/g pro;正常对照组为88.43U/g pro,两组比较,差异有统计学意义;高剂量染毒组肝脏中抗超氧阴离子为190.82U/g pro;正常对照组为329.29U/g pro,两组比较,差异有统计学意义;低剂量染毒组肾脏中抗超氧阴离子为285.05U/g pro,正常对照组为120.03U/g pro,两组比较,差异有统计学意义;低、高剂量染毒组脑组织中抗超氧阴离子分别为171.86和218.05U/g pro,与正常对照组(285.05U/g pro)比较,差异均有统计学意义。结论羰基镍引起不同脏器的氧化应激和氧化损伤,且存在剂量和器官差异。
OBJECTIVE: To investigate the changes of anti-superoxide anion content in various organs of acute carbonyl nickel poisoning rats and to explore the toxic effects and micro-mechanism of nickel carbonyl poisoning in rats. Methods SD healthy rats were exposed to static inhalation, nickel carbonyl dose was 20,135,250 mg / m3, the chlorine dose of the control group was 250mg / m3, the exposure time was 30min, and the normal control group , 1, 2, 3 and 7 days after exposure. The content of anti-superoxide anion was determined by chemical colorimetry. Results In the first day of exposure, the anti-superoxide anion in the lung was 119.70U / g pro and in the normal control group was 88.43U / g pro, the difference was statistically significant Anti-superoxide anion in the liver of the group was 190.82U / g pro and 329.29U / g pro in the normal control group, the difference was statistically significant. In the low dose group, the anti-superoxide anion in the kidney was 285.05U / g pro, the normal control group was 120.03U / g pro, the difference between the two groups was statistically significant; low and high dose of the brain tissue anti-superoxide anion were 171.86 and 218.05U / g pro, respectively, compared with the normal control group (285.05U / g pro), the differences were statistically significant. Conclusion Carbonyl nickel causes oxidative stress and oxidative damage in different organs, and there are dose and organ differences.