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为了探讨自体角朊细胞体外对人-猪异种混合血管内皮细胞-淋巴细胞反应(MELR)的影响及作用机制,我们从mR-NA;蛋白水平观察淋巴细胞的增殖;细胞因子的分泌及胞内细胞因子的变化,并用IL-10单抗进行封阻,观察其对角朊细胞诱导免疫抑制效应的影响。结果发现在MELR中引入自体角朊细胞后,细胞因子分泌格局呈现由IFN-γ主导转变为由IL-10主导的动力学变化;IL-10单抗能有效地阻断角朊细胞所诱导的免疫抑制效应。表明自体角朊细胞可诱导一群分泌IL-10的调节性T细胞,从而抑制Th1亚群所介导的细胞免疫及异种移植物排斥反应。
To investigate the effect of autologous keratinocytes on human-porcine xeno-mixed vascular endothelial cell-lymphocyte reaction (MELR) in vitro and its mechanism of action, we observed the proliferation of lymphocytes from mR-NA; protein levels; the secretion of cytokines and intracellular Cytokine changes, and blocking with IL-10 monoclonal antibody to observe the keratinocyte-induced immune suppression effect. As a result, it was found that the introduction of autologous keratinocytes into MELR resulted in a change in the cytokine secretion pattern dominated by IL-10 from IFN-γ-induced IL-10. IL-10 mAb effectively blocked keratinocyte-induced Immunosuppressive effect. This suggests that keratinocytes induce a population of regulatory T cells that secrete IL-10, thereby inhibiting the Th1 subpopulation-mediated cellular immunity and xenograft rejection.