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目的研究PAK5对大肠癌细胞黏附以及迁移的影响。方法采用基因转染上调PAK5在LST细胞中的表达水平,以及RNA干扰下调PAK5在SW480细胞中的表达水平,采用细胞黏附以及迁徙实验检测PAK5的黏附、迁徙能力;结果采用基因转染上调PAK5在LST细胞株中的表达水平后,细胞-基质黏附能力减弱,迁徙能力增强;相反,采用RNA干扰敲低PAK5在SW480细胞株中的表达水平后,细胞-基质黏附能力增强,迁徙能力减弱。结论PAK5通过减弱细胞-基质黏附,增强大肠癌细胞的迁徙能力。
Objective To study the effect of PAK5 on the adhesion and migration of colorectal cancer cells. Methods The expression of PAK5 in LST cells was induced by gene transfection and the expression of PAK5 in SW480 cells was down-regulated by RNA interference. The adhesion and migration of PAK5 were detected by cell adhesion and migration assay. RESULTS: LST cells, the cell-matrix adhesion ability and migratory ability were enhanced. On the contrary, knockdown of PAK5 expression in SW480 cells by RNA interference enhanced the cell-matrix adhesion capacity and migration ability. Conclusion PAK5 enhances the migration of colorectal cancer cells by attenuating cell-matrix adhesion.