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目的 :探讨新型肝炎相关病毒 ( TTV)在慢性非甲—庚型肝病患者中的感染状况 ,分析国内病毒的基因结构特点。方法 :根据国内外报道的部分基因序列 ,自行设计特异性引物 ,采用聚合酶链反应 ( PCR)法检测了 50例慢性非甲—庚型肝病患者 ,并对阳性标本克隆后进行序列分析。结果 :慢性非甲—庚型肝病患者中 TTV感染率为 52 % ,对 TTV病毒株进行序列分析发现与N2 2克隆同源性可达 97.8%。结论 :我国也存在严重的 TTV感染 ,TTV可能为慢性非甲—庚型肝病的致病因子。我国 TTV感染株与国外报道的 N2 2克隆有高度同源性。
Objective: To investigate the infection status of novel hepatitis-related virus (TTV) in patients with chronic non-G-hepatopathy and to analyze the gene structural characteristics of domestic virus. Methods: According to the partial gene sequences reported at home and abroad, specific primers were designed by ourselves. 50 patients with chronic non-Hepatitis G virus were detected by polymerase chain reaction (PCR), and the positive samples were cloned and sequenced. Results: The prevalence of TTV infection was 52% in patients with chronic non-A-G liver disease. Sequence analysis of the TTV virus showed 97.8% homology with N2 2 clone. Conclusion: There is also a serious TTV infection in China. TTV may be the causative agent of chronic non-A-G liver disease. TTV infection in China and foreign reports of N2 2 clones have a high degree of homology.