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目的:在耐药胃癌细胞系SGC7901/ADR中对Ro60进行克隆,对Ro60在耐药胃癌细胞系SGC7901/ADR和药敏胃癌细胞系SGC7901中的表达情况进行比较分析。方法:应用RT-PCR法克隆Ro60的编码基因,通过DNA序列测定对所克隆的基因进行验证。应用半定量RT-PCR法检测Ro60在耐药胃癌细胞系SGC7901/ADR和药敏胃癌细胞系SGC7901中的表达情况。结果:在耐药胃癌细胞系SGC7901/ADR中,成功克隆了Ro60的编码基因,其DNA序列与Ro60的cDNA序列完全一致。半定量RT-PCR结果显示,Ro60在耐药胃癌细胞系SGC7901/ADR中的表达强度显著高于药敏胃癌细胞系SGC7901细胞中的表达强度,P<0.01。结论:Ro60在耐药胃癌细胞系中高表达,该分子可能是一种胃癌多药耐药相关分子。
OBJECTIVE: To clone Ro60 in drug-resistant gastric cancer cell line SGC7901 / ADR and compare the expression of Ro60 in drug-resistant gastric cancer cell line SGC7901 / ADR and drug-resistant gastric cancer cell line SGC7901. Methods: The gene encoding Ro60 was cloned by RT-PCR and the cloned gene was verified by DNA sequencing. The expression of Ro60 in drug-resistant gastric cancer cell line SGC7901 / ADR and drug sensitive gastric cancer cell line SGC7901 was detected by semi-quantitative RT-PCR. Results: The gene encoding Ro60 was successfully cloned in the resistant gastric cancer cell line SGC7901 / ADR. The DNA sequence of Ro60 was exactly the same as that of Ro60. Semi-quantitative RT-PCR results showed that the expression level of Ro60 in drug-resistant gastric cancer cell line SGC7901 / ADR was significantly higher than that in drug-sensitive gastric cancer cell line SGC7901, P <0.01. Conclusion: Ro60 is overexpressed in drug-resistant gastric cancer cell lines, which may be a multidrug resistance-related molecule in gastric cancer.