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目的:研究CPT-11对食管癌细胞株ECa-109细胞周期及其放射敏感性的影响。方法:将ECa-109细胞株分为单纯培养组(对照组)、单纯化疗组、单纯放疗组、同时化放疗及时相化放疗组;MTT法检测细胞存活率,选择CPT-11的最佳实验浓度;流式细胞仪(FCM)观察CPT-11诱导ECa-109细胞阻滞于G2期的时间,作为时相化放疗组加药后行放疗的时间点,并分析各组细胞的凋亡率;克隆形成实验观察CPT-11作用后各放疗组细胞的存活率。结果:3.85μg/mL CPT-11为最佳实验浓度;FCM检测细胞周期显示,细胞处于G2期的时间点约为加药后12 h;FCM检测细胞凋亡显示,化疗组、放疗组、同时化放疗组、时相化放疗组疗效依次递增P<0.05;化疗组与对照组差异无统计学意义,P>0.05;细胞克隆形成实验显示,CPT-11能降低ECa-109放疗后存活率;时相化放疗组及同时化放疗组均优于单纯放疗组,且前者优于后者;两者的放疗增敏比分别为1.39和1.11。结论:CPT-11对ECa-109细胞株有放射增敏作用且时相化放疗的增敏作用更强。
Objective: To study the effect of CPT-11 on the cell cycle and radiosensitivity of esophageal cancer cell line ECa-109. Methods: ECa-109 cell lines were divided into simple culture group (control group), simple chemotherapy group, radiotherapy alone group, simultaneous radiotherapy and concurrent phase radiotherapy group; MTT assay cell survival rate, the best choice of CPT-11 (FCM) was used to observe the time of CPT-11-induced ECa-109 cell arrest in G2 phase as the time point of radiotherapy in the time-phased radiotherapy group and the apoptosis rate The clonogenic assay was used to observe the survival rate of each radiotherapy group after CPT-11 treatment. Results: 3.85μg / mL CPT-11 was the best experimental concentration. The cell cycle of FCM assay showed that the cell cycle in G2 phase was about 12 h after dosing; FCM showed that the apoptosis rate in chemotherapy group, radiotherapy group, Chemotherapy group and control group showed no significant difference (P> 0.05). Cell clonogenic assay showed that CPT-11 could reduce the survival rate of ECa-109 after radiotherapy; Time-phased radiotherapy group and concurrent radiotherapy group were better than radiotherapy alone group, and the former was better than the latter; the radiosensitivity of the two groups were 1.39 and 1.11 respectively. CONCLUSION: CPT-11 has a radiosensitizing effect on ECa-109 cell line and the sensitizing effect of phase-contrast radiotherapy is stronger.