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目的探讨FOXP3 m RNA荧光定量PCR技术在继发性肺结核患者疗效监测的应用意义。方法研究组选取继发性肺结核病人47例,收集初诊痰液,染色和镜检。选取健康对照40例。分离两组淋巴细胞,利用FOXP3 m RNA定量PCR技术分析对照组和研究组0周及治疗后2周、4周和8周时的FOXP3 m RNA表达情况,应用SPSS16.0软件统计分析实验数据。结果 0周时47名研究组抗酸染色均为阳性,对照组均为阴性。以抗酸染色结果分组,抗酸(+)组、抗酸(++)组、抗酸(+++)组和抗酸(++++)组与对照组FOXP3 m RNA表达差异均有统计学意义(P<0.05,P<0.01)。治疗时间对抗酸染色各组FOXP3 m RNA表达水平有影响(P<0.01),治疗时间和抗酸各组因素存在交互作用(P<0.01)。研究组和对照组FOXP3 m RNA表达差异有统计学意义(P<0.01)。研究组治疗不同时间点方差分析显示治疗时间对研究组FOXP3 m RNA表达水平有影响(P<0.01)。结论 FOXP3 m RNA定量PCR检测敏感,特异,可以用于继发性肺结核患者疗效和其机体免疫力的监测。
Objective To investigate the significance of FOXP3 m RNA quantitative PCR in monitoring the curative effect of patients with secondary pulmonary tuberculosis. Methods Study group selected 47 cases of secondary pulmonary tuberculosis, collected sputum, staining and microscopic examination. Select healthy control in 40 cases. The two groups of lymphocytes were separated and the expression of FOXP3 mRNA in control group and study group was analyzed by FOXP3 m RNA quantitative PCR at 0 week, 2 weeks, 4 weeks and 8 weeks after treatment. The experimental data were analyzed by SPSS16.0 software. Results At the 0th week, all 47 study groups were positive for acid-fast staining while the control group were negative. The expression of FOXP3 mRNA in the groups of antacid (+), antacid (++), antacid (+++) and antacid (++++) and control groups was Statistical significance (P <0.05, P <0.01). The treatment time had an effect on the expression of FOXP3 mRNA (P <0.01), and the interaction between the treatment time and each group of antacids (P <0.01). The FOXP3 mRNA expression in study group and control group was significantly different (P <0.01). Analysis of variance at different time points in the study group showed that the treatment time had an impact on the FOXP3 mRNA expression in the study group (P <0.01). Conclusion FOXP3 m RNA quantitative PCR detection is sensitive and specific, which can be used to monitor the efficacy and immunity of the patients with secondary pulmonary tuberculosis.