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目的定量研究细胞间粘附分子基因(ICAM—1 mRNA)在不同病理类型颅咽管瘤的表达差异及意义。方法收集30例经手术治疗的颅咽管瘤标本,采用SYBR荧光实时定量PCR法检测 ICAM-1 mRNA在肿瘤组织的表达,并对表达结果行统计学分析。结果造釉细胞型颅咽管瘤 ICAM-1mRNA表达量为(62．18±6．43)×103 copies／μg,鳞状乳头型颅咽管瘤ICAM-1 mRNA表达量为 (1．13±0．17)×103 copies／μg,造釉细胞型颅咽管瘤ICAM-1 mRNA表达量显著性高于鳞状乳头型颅咽管瘤(P<0．01)。结论两种病理类型颅咽管瘤ICAM—1 mRNA表达存在显著性差异,此差异性可能与两种病理类型颅咽管瘤不同的肿瘤炎症有关。
Objective To quantitatively study the expression and significance of intercellular adhesion molecule (ICAM-1) mRNA in different pathological types of craniopharyngiomas. Methods Thirty surgical specimens of craniopharyngioma were collected. The expression of ICAM-1 mRNA in the tumor tissue was detected by SYBR real-time quantitative PCR, and the expression was analyzed statistically. Results The expression of ICAM-1 mRNA in glaucomatous craniopharyngioma was (62.18 ± 6.43) × 103 copies / μg, and the expression of ICAM-1 mRNA in squamous nipple craniopharyngioma was (1.13 ± 0.17) × 103 copies / μg, the expression of ICAM-1 mRNA in glaucoma craniopharyngioma was significantly higher than that in squamous papillary craniopharyngioma (P <0.01). Conclusion The expression of ICAM-1 mRNA in two types of craniopharyngiomas is significantly different, which may be related to different tumor types in two types of craniopharyngiomas.