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为探讨即刻早期基因在预处理中的作用机制 ,将培养心肌细胞分别经短暂缺血及佛波酯、去甲肾上腺素、腺苷预处理 ,行模拟缺血再灌注 ,采用Northern杂交技术对心肌细胞c fos、c junmRNA含量进行检测。结果发现 ,缺血预处理组c fos、c junmRNA明显上升 ,其他药物预处理组c fos、c jun基因均有不同程度的表达增强 ,H7能明显抑制预处理对c fos、c junmRNA的诱导。表明预处理可能通过激活PKC ,进而诱导c fos、c jun基因的转录表达增强 ,PKC可能在缺血预处理中起着关键的作用
In order to explore the mechanism of immediate early gene in pretreatment, myocardial cells were transiently ischemic and phorbol ester, norepinephrine, adenosine preconditioning respectively, and simulated ischemia-reperfusion. Cell c fos, c junmRNA content was detected. The results showed that the expression of c fos and c jun mRNA increased significantly in ischemic preconditioning group. The expressions of c fos and c jun genes in other pretreatment groups were enhanced to different extents. H7 significantly inhibited the induction of c fos and c jun mRNA. These results suggest that preconditioning may play an important role in ischemic preconditioning by activating PKC and inducing the transcriptional expression of c fos and c jun genes.