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对抗枣疯病婆枣进行组织培养 ,若在 6月中旬采集外植体 ,其茎段灭菌时间可以采用 70 %酒精 4 0S +0 .1 %升汞 1 0～ 1 2min ,或 70 %酒精 1 0S +0 .1 %升汞 1 2min ,成活率可达 4 3%～ 4 7%。用茎尖作外植体 ,其灭菌时间可以采用70 %酒精 1 0S +0 .1 %升汞 5min ,成活率达 33.33%。托叶及残留叶柄的真菌污染率很高 ,在前处理阶段把托叶及残留叶柄去掉 ,可有效降低污染率。
If the explants are collected in mid-June, the sterilization time of the stem segments can be 70% alcohol 40% + 0.1% mercuric chloride 10 ~ 12min, or 70% alcohol 1 0S +0 .1% mercuric chloride 1 2min, the survival rate of up to 43% ~ 47%. With the tip of the shoots as explants, the sterilization time can be 70% alcohol 10S +0. 1% mercuric chloride 5min, the survival rate of 33.33%. Stipules and residual petiole fungal contamination rate is very high, in the pre-treatment stage to remove the leaves and residual petiole, which can effectively reduce the pollution rate.