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为明确引起福建省福州地区豨莶上黄脉症状的病原,利用PCR技术,从3个样品上均扩增到双生病毒约500 bp的特异片段,选择病毒分离物Fz02进行全序列测定.结果表明:Fz02 DNA-A全长2771 nts,具有典型的双生病毒的基因组结构特征,与豨莶黄脉病毒广州分离物(Sb YVV-Gz01)同源性最高,达93.4%.利用设计的卫星分子的特异性引物,在病毒分离物Fz02中扩增到betasatellite分子(Fz02β).Fz02β全长1359 nts,与Sb YVV广东分离物(GD13)伴随的betasatellite同源性最高,为85.3%.系统关系树表明,Fz02与Sb YVV各分离物聚类为一个大分支.此外,Sb YVV序列变异较大.
In order to elucidate the etiopathogenisis of Huangmai in Fuzhou of Fujian Province, a specific fragment of about 500 bp was amplified from all three samples by polymerase chain reaction (PCR), and the full-length sequence of the virus was selected by Fz02 : Fz02 DNA-A has a total length of 2771 nts and has the typical genomic structural features of the geminiviruses with the highest homology of 93.4% with the yellowish vein virus Guangzhou isolate (Sb YVV-Gz01) (Fz02β). Fz02β was 1359 nts in length and had the highest homology (85.3%) with the betasatellite associated with Guangdong isolate (GD13) of Sb YVV, and the phylogenetic tree showed that , Fz02 and Sb YVV clustered into a large branch of each branch.In addition, Sb YVV sequence variation.