论文部分内容阅读
目的以鱼腥草为材料,研究鱼腥草蛋白酶抑制剂(HCTI)的分离提取工艺。方法 HCTI的最佳粗提条件是采用pH7.0的双蒸水为提取溶剂,在体积比为1:30,45℃条件下浸提2.0h,提取2次。硫酸铵部分沉淀后,再经DEAE-52离子交换层析柱纯化。结果粗提液经45%～60%饱和度的硫酸铵沉淀比活力最高,经过DEAE-52离子交换层析柱梯度洗脱后,检测到HCTI活性峰。结论用DEAE-52离子交换层析柱可分离纯化HCTI,SDS-聚丙烯酰胺凝胶电泳,计算出该HCTI的分子量约为28KDa。
OBJECTIVE To study the isolation and extraction of Houttuynia cordata (Houttuynia) enzyme inhibitor (HCTI) from Houttuynia cordata Thunb. Methods The optimum extraction conditions for HCTI were extraction with double distilled water (pH7.0) as extraction solvent at a volume ratio of 1: 30 and 45 ℃ for 2.0 h and extraction twice. Ammonium sulfate was partially precipitated and then purified by DEAE-52 ion exchange chromatography. Results The crude extract had the highest specific activity of precipitating ammonium sulfate with 45% ~ 60% saturation. The peak of HCTI activity was detected after gradient elution through DEAE-52 ion exchange chromatography. Conclusion DEAE-52 ion exchange chromatography column can be separated and purified by HCTI, SDS-polyacrylamide gel electrophoresis. The calculated molecular weight of the HCTI is about 28KDa.