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目的研究旋毛虫肌幼虫寄生时间对其感染力的影响。方法人工消化收集旋毛虫幼虫,35只昆明小鼠每只口饲30条幼虫。感染鼠随机分为6组,分笼喂养,分别于感染后至1、2、4、6、8、10个月,用同样方法收集幼虫;另取10只健康小鼠,每只口饲30条幼虫。感染鼠于2个月后全部断颈处死,取胴体称重,取膈肌镜检,消化收集小鼠体内全部旋毛虫幼虫,计算生殖力指数(RCI)、每克组织肌幼虫数(LPG)。结果感染后2个月,5只小鼠RCI和LPG分别为108.17和181.59,134.17和189.86,114.17和181.12,121.80和198.05,146.13和186.32。感染后4、6、8个月,旋毛虫RCI和LPG逐渐降低。各组小鼠RCI和LPG差异均有统计学意义(P<0.05)。结论旋毛虫感染力随寄生时间变化,感染后2个月达高峰,之后随寄生时间的延长,感染力逐渐下降。
Objective To study the effect of parasitological time of Trichinella spiralis larvae on its infectivity. Methods Trichinella larvae were collected by artificial digestion, and 35 larvae were inoculated in 35 Kunming mice respectively. Infected rats were randomly divided into 6 groups, fed in separate cages. The larvae were collected by the same method at 1, 2, 4, 6, 8 and 10 months after infection respectively. Another 10 healthy mice were fed with 30 Larva The mice were sacrificed at 2 months after sacrifice. The carcasses were weighed, and all mice in the testis were collected by digestion and digestion. The reproductive index (RCI), number of muscle larvae per gram (LPG) were calculated. Results At 2 months after infection, the RCI and LPG of 5 mice were 108.17 and 181.59, 134.17 and 189.86, 114.17 and 181.12, 121.80 and 198.05, 146.13 and 186.32, respectively. At 4, 6 and 8 months after infection, Trichinella RCI and LPG gradually decreased. The differences of RCI and LPG in each group were statistically significant (P <0.05). Conclusion The infectivity of Trichinella spiralis varied with the parasitism time and reached the peak at 2 months after infection, and then decreased with parasitization time.