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来自木荷叶和无患子中果皮的皂甙抽提物对抗稻瘟病原活性被试验。皂甙抽提物体外抑制活性显示了皂甙剂量与抗真菌效果高度相关性。它们的抗稻瘟有效中浓度EC50:木荷叶乙醇抽提物为42.95μg/mL,木荷叶水抽提物为452.91μg/mL,无患子中果皮的甲醇抽提物为95.65μg/mL。一个重要的结果是当上述木荷叶和无患子中果皮醇抽提物以3∶4到15∶8的质量配比时产生了显著的增效抗稻瘟作用,而在其它配比时有相加作用。通过乙醚/丙酮沉析、硅胶柱色谱分离,以及酸水解和薄层过程,检测出木荷抽提物皂甙中含有2种三萜类皂甙元和5种单糖。纯化后的皂甙在150μg/mL时显示了98.28%的抑制率。此研究结果表明:木荷叶抽提物,或木荷叶和无患子中果皮抽提复配物对毁灭性的稻瘟病害具有重要地抗病效果。
Saponins from Schima superba and Sapindaceae saponin extracts were tested against blast pathogens. In vitro inhibitory activity of saponin extract showed a high correlation between saponin dose and antifungal effect. Their anti-rice blast effective concentration of EC50: Schima superba ethanol extract of 42.95μg / mL, Schima superba water extract of 452.91μg / mL, Sapindus caroti methanol extract was 95.65μg / mL. An important result is that when the peel alcohol extract of the Schisandra chinensis and Sapindus pilosulae produced a significant synergistic anti-rice blast at a mass ratio of 3: 4 to 15: 8, at other compounding times Have additive effect. The triterpene saponins of two kinds of triterpenoid saponins and five kinds of monosaccharides were detected by ether / acetone precipitation, silica gel column chromatography, acid hydrolysis and thin layer process. The purified saponin showed a 98.28% inhibition at 150 μg / mL. The results of this study show that the extracts of M. oryzae or the peel extract of S. japonica and S. japonicus have an important resistance to the devastating rice blast disease.