论文部分内容阅读
目的:探讨不同胎龄新生儿早期血清神经特异性蛋白的变化及生后日龄对神经特异性蛋白的影响,评价这些指标在新生儿脑成熟度方面的临床意义。方法:84例不同胎龄的正常新生儿,按胎龄分为:Ⅰ、Ⅱ、Ⅲ、Ⅳ组,其胎龄分别为28-31,32-34,35-36和37周及以上。所有观察对象于生后2-3d以酶联免疫吸附试验测定血清神经特异性烯醇化酶(NSE)、S100蛋白(S100)、髓鞘碱性蛋白(MBP)。对其中27例新生儿于出生7d后再次测定。结果:①Ⅰ、Ⅱ、Ⅲ、Ⅳ组的血清NSE、MBP、S100水平无显著性差异(P>0.05)。②出生3d内及7d后血清MBP水平无显著性差异(P>0.05),而NSE、S100不同日龄差异均有显著性意义(P值分别为0.018,0.016)。③MBP、NSE与胎龄无明显相关性,S100与胎龄轻度负相关(r=-0.270,P=0.013)。结论:①S100血清浓度受新生儿胎龄的影响,而NSE、MBP与胎龄无关。②NSE、S100血清浓度随生后日龄不同而变化,MBP则相对稳定。
OBJECTIVE: To investigate the changes of serum specific neuroproteins in early neonates with different gestational age and the effect of postnatal day-old on neural-specific proteins, and to evaluate the clinical significance of these indexes in neonatal brain maturity. Methods: Eighty-four normal newborn infants of different gestational ages were divided into groups Ⅰ, Ⅱ, Ⅲ and Ⅳ according to gestational age. Their gestational ages were 28-31, 32-34, 35-36 and 37 weeks, respectively. All subjects observed serum neuron specific enolase (NSE), S100 protein (S100), and myelin basic protein (MBP) by enzyme-linked immunosorbent assay 2-3 days after birth. Of these, 27 newborns were tested again 7 days after birth. Results ① There was no significant difference in serum NSE, MBP and S100 between Ⅰ, Ⅱ, Ⅲ and Ⅳ groups (P> 0.05). There were no significant differences in serum MBP levels within 3d and 7d after birth (P> 0.05), but there were significant differences in NSE and S100 between different days (P = 0.018 and 0.016, respectively). ③MBP, NSE had no significant correlation with gestational age, S100 mildly negative correlation with gestational age (r = -0.270, P = 0.013). Conclusion: ①S100 serum concentration is influenced by gestational age, while NSE and MBP have no relation with gestational age. ②NSE, S100 serum concentrations vary with the age of the day after birth, MBP is relatively stable.