鼻咽癌组织中人疱疹病毒6型感染及其临床意义

• 作 者: 陈心春 简少文 汪慧民 曾木圣
• 机 构: 深圳市东湖医院,中山医科大学肿瘤研究所,中山医科大学肿瘤研究所,中山医科大学肿瘤研究所
• 出 处: 中华实验和临床病毒学杂志
• 发表日期: 1999年03期
• 关键词: 鼻咽癌组织 疱疹病毒 癌前病变组织 LMP1 EB病毒 人疱疹病毒6型 聚合酶链反应 免疫组化检测 异型增生 原位杂交

目的　探讨人疱疹病毒６ 型（ＨＨＶ６） 与鼻咽癌（ＮＰＣ） 发病的关系。方法　采用聚合酶链反应（ＰＣＲ） 和原位杂交同时检测正常鼻咽组织和ＮＰＣ 癌前鼻咽组织以及ＮＰＣ 组织中ＨＨＶ６ 和ＥＢＶＤＮＡ 的存在情况。采用免疫组化检测３６ 例ＮＰＣ 组织的ＥＢＶＬＭＰ１ 蛋白表达。结果　在４２ 例ＮＰＣ组织、２１ 例鼻咽癌前病变组织中，经ＰＣＲ检出ＨＨＶ６ ＤＮＡ，阳性率分别为３０－９％ （１３ 例） 和４－７ ％（１ 例） 。ＥＢＶＤＮＡ为９５－２％ （４０ 例） 和６６－６ ％ （１４ 例）。２７ 例正常鼻咽组织未检出ＨＨＶ６ ＤＮＡ，ＥＢＶＤＮＡ为２５－９％ （７ 例）。经ＩＳＨ，仅在１３ 例ＰＣＲＨＨＶ６ ＤＮＡ阳性的ＮＰＣ组织中检出１１ 例阳性，３６ 例ＮＰＣ组织、１０ 例鼻咽癌前病变组织ＩＳＨＥＢＶＤＮＡ 阳性。ＮＰＣ组织的ＥＢＶＬＭＰ１ 蛋白阳性率为４７－２ ％（１７３６）。结论　鼻咽癌组织中存在ＨＨＶ６ 感染，提示ＨＨＶ６ 感染与ＮＰＣ有关。ＨＨＶ６ 感染与ＥＢ病毒ＬＭＰ１ 蛋白表达上调呈正相关，提示在ＮＰＣ 的发生中ＨＨＶ６ 可能直接参与和（ 或） 通过上调ＥＢＶＬＭＰ１ 的表达而起一定作用

Objective To investigate the relationship between human herpes virus type 6 (HHV6) and nasopharyngeal carcinoma (NPC). Methods Polymerase chain reaction (PCR) and in situ hybridization were used to detect the presence of HHV6 and EBVDNA in normal nasopharyngeal tissues, NPC pre-NPC tissues and NPC tissues simultaneously. Immunohistochemistry was used to detect EBVLMP1 protein expression in 36 cases of NPC. Results In 42 NPC tissues and 21 nasopharyngeal precancerous lesions, the positive rate of HHV6 DNA was 30-9% (13 cases) and 4-7% (1 case) by PCR. EBVDNA was 95-2% (40 cases) and 66-6% (14 cases). 27 cases of normal nasopharyngeal tissue were not detected HHV 6 DNA, EBV DNA was 25-9% (7 cases). Eleven cases were positive, 36 cases were NPC tissues, and 10 cases were positive for ISHEBVDNA in nasopharyngeal precancerous lesions in only 13 cases of PCRHHV6 DNA positive NPC tissues. The positive rate of EBVLMP1 protein in NPC was 47-2% (1736). Conclusion The presence of HHV 6 in nasopharyngeal carcinoma tissue infection, suggesting that HHV 6 infection and NPC. HHV 6 infection and Epstein-Barr virus LMP1 protein expression was positively correlated, suggesting that in the occurrence of NPC HHV 6 may be directly involved and (or) by up-regulating the expression of EBVLMP1 play a role