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目的:利用Platelia酶联免疫试剂盒直接测定支气管肺泡灌洗液中的半乳甘露聚糖(GM),评价该方法的实用性和有效性。方法:回顾性分析临床疑诊IFI住院患者137例,确诊或临床诊断患者15例。结果:阳性界值为0.5时,支气管肺泡灌洗液中GM检测的敏感性、特异性、阳性预测值、阴性预测为100%、85.5%、62.5%、100%。阳性界值为1.0时支气管肺泡灌洗液中GM检测的敏感性、特异性、阳性预测值、阴性预测为100%、96.8%、88.2%、100%。结论:支气管肺泡灌洗液样本的GM检测对于IFI患者是安全和有效的辅助诊断手段。
OBJECTIVE: To directly determine the galactomannan (GM) in bronchoalveolar lavage fluid by Platelia enzyme-linked immunosorbent assay (ELISA), and evaluate its practicability and effectiveness. Methods: Retrospective analysis of 137 cases of clinical suspected IFI inpatients, diagnosed or clinically diagnosed in 15 patients. Results: The sensitivity, specificity and positive predictive value of GM in bronchoalveolar lavage fluid were 100%, 85.5%, 62.5% and 100% respectively when the positive cutoff value was 0.5. The sensitivity, specificity and positive predictive value of GM in bronchoalveolar lavage fluid were 100%, 96.8%, 88.2% and 100% respectively when the positive cutoff value was 1.0. Conclusion: The GM detection of bronchoalveolar lavage fluid samples is safe and effective in the diagnosis of IFI.