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为了配合初中生理卫生循环系统一章教学,可以进行离体蛙心灌流的演示实验,帮助学生更直观了解心脏机能。在大学生理实验室常用八木氏插管和斯氏插管进行蛙心灌流实验。我们则利用一只眼药瓶和一段普通细塑胶管,制成简易的灌流器。此仪器装置简单,效果又好。适合于一般中学教学。实验材料眼药水瓶,细塑料胶管(空心的,扎小辫用的);化学实验用铁支架,简易杠杆(参阅生物学通报1960年3月号),简易记纹板,任氏溶液,蟾蜍(或蛙)。实验方法 (1)灌流器的制作:把眼药瓶的细口在酒精灯火焰上加热。加热时应集中在一圈,当玻璃
In order to cooperate with junior high school physiology health circulatory system chapter teaching, you can carry out demonstration experiments of frog heart perfusion to help students more intuitive understanding of cardiac function. Commonly used Yagi’s intubation and Stein’s intubation in the university physiology laboratory for frog heart perfusion experiments. We use an eye bottle and a section of ordinary fine plastic hose, made of simple perfusion device. This device is simple, good effect. Suitable for general secondary education. Experimental materials Eye drops bottles, thin plastic hose (hollow, with pigtails); chemical experiments with iron brackets, simple lever (see Bulletin of Biology in March 1960), simple note plate, Ren’s solution, toad Or frog). Experimental methods (1) the production of the perfusion device: the eyedropper bottle in the alcohol lamp flame heating. Heating should be concentrated in a circle, when the glass