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利用玉米雄性不育C组的5种胞质(BB、C,ES、PR、RB):在两种遗传背景下(A619和A632),限朋了在一组相近的胞质中线粒体基因组多样性的性质。利用携带来自玉米N和S胞质重组活跃的单拷贝序列柯斯质粒和质粒作为探针。尽管这5种胞质的限制性图谱都极为相似,但是,根据序列的重复和重组夕来自N细月诃质重组活跃序列的缺失,小型环状DNA的数量以及限制性图谱,可将这5种胞质区别开来。该组内每种胞质都携带有l.913bp的小型环状线粒体DNA(mt DNA),除了RB以外,还携带有l.445bP的小型环状线粒体DNA,这说明C组在分子水平上并不是完全一致的,该组的进化包括主基因组的重组,涉及序列的重复和缺失,但是,对细胞质雄性不育性没有影响。
Five cytoplasmic (BB, C, ES, PR, RB) groups of maize male sterile C were used: in both genetic backgrounds (A619 and A632), mitochondrial genome diversity was similar in a group of similar cytoplasm Sexual nature. A single copy of the cosmid and plasmid carrying the cytoplasmic recombinant active from N and S was used as a probe. Although the five cytoplasmic restriction maps are very similar, based on the sequence repeats and recombination events, the absence of the N-terminus reorganization active sequence, the number of small circular DNA, and the restriction map, Differentiate the cytoplasm. Each cytoplasm in this group carries a small l.913 bp small circular mitochondrial DNA (mt DNA) carrying, in addition to RB, small l445bP small circular mitochondrial DNA, which indicates that group C is on the molecular level and Not entirely identical, the group’s evolution includes the recombination of the main genome, involving sequence repeats and deletions, but has no effect on cytoplasmic male sterility.