论文部分内容阅读
红细胞生成素(Erythropoicun,Ep)已被确认为主要造血调节因子。1977年人Ep被纯化,不久人Ep基因被克降和表达。重组人Ep的结构和生物特性与天然Ep无显著不同。人Ep为酸性糖蛋白,分子量约34.000,其蛋白质部份包含166个氨基酸残基,其糖组成部份,特别是唾液酸部份,与Ep生物活性的发挥有重要关系,失去唾液酸部份的Ep迅速被肝从血内清除。Ep不能通过啮齿类和羊的胎盘,但初生鼠可经哺乳而获得Ep。 Ep促原始红细胞增殖,分化和成熟。较原始的红系细胞(如BFU-E)不仅受Ep控制,也接受IL-3,GM-CSF等其他造血因子的影响,而稍成熟一些的原始红细胞(如CFU-E)主要受Ep控
Erythropoietin (Ep) has been identified as the major hematopoietic regulator. Human Ep was purified in 1977, and soon the human Ep gene was knocked down and expressed. The structure and biological properties of recombinant human Ep are not significantly different from that of native Ep. Ep is an acidic glycoprotein with a molecular weight of about 34.000 and a protein portion of 166 amino acid residues. The sugar moiety, especially the sialic acid moiety, plays an important role in the bioactivity of Ep, losing the sialic acid moiety Ep quickly cleared by the liver from the blood. Ep does not pass through the placenta of rodents and sheep, but newborns get Ep after lactation. Ep promotes primitive erythrocyte proliferation, differentiation and maturation. Compared with original erythroid cells (such as BFU-E), it is not only controlled by Ep, but also influenced by other hematopoietic factors such as IL-3 and GM-CSF. Some mature erythrocytes (such as CFU-E)