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目的:探讨螺内酯对大鼠心肌缺血再灌注损伤时细胞凋亡的影响。方法:选择30只健康SD大鼠,采用结扎冠状动脉左前降支后再通的方法复制心肌缺血再灌注的动物模型,随机分为三组:假手术组(n=10)、缺血再灌注(MI/RI)组(n=10)和药物组(n=10)。光镜及电镜观察标本病理形态改变,检测各组再灌注3h后心肌局部凋亡相关因子bcl-2、fas的表达,比较各组间差异。结果:与假手术组比较,MI/RI组中Fas含量升高(P>0.01),Bcl-2含量明显减少(P>0.05);药物组较MI/RI组Fas含量明显降低(P>0.05),Bcl-2含量明显增加(P>0.05)。结论:螺内酯减轻MI/RI中心肌细胞凋亡程度。
Objective: To investigate the effect of spironolactone on apoptosis in myocardial ischemia-reperfusion injury in rats. Methods: Thirty healthy SD rats were randomly divided into three groups: sham-operated group (n = 10), ischemia-reperfusion group Perfusion (MI / RI) group (n = 10) and drug group (n = 10). The pathological changes of the specimens were observed under light microscope and electron microscope. The expression of bcl-2 and fas in myocardium were detected after 3 hours of reperfusion, and the differences among groups were compared. Results: Compared with sham operation group, the content of Fas in MI / RI group increased (P> 0.01) and the content of Bcl-2 decreased significantly (P> 0.05) ), Bcl-2 content increased significantly (P> 0.05). Conclusion: Spironolactone can reduce the MI / RI cardiomyocyte apoptosis.