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目的检测病毒灭活处理血浆中亚甲蓝(methylene blue,MB)残留量。方法将4家采供血机构(A、B、C、D)提供的血浆样品经2个不同型号的一次性病毒灭活装置处理后,采用固相萃取方法分别提取病毒灭活血浆及标准品中MB,利用高效液相色谱法检测MB含量。结果 4家采供血机构病毒灭活血浆MB平均残留量分别为(0.29±0.11)、(0.26±0.09)、(0.09±0.03)和(0.10±0.02)μmol/L。两种不同型号滤器对MB的滤除率分别为91%和73%,差异有统计学意义(P<0.01)。结论目前我国病毒灭活血浆中MB残留量基本符合质量要求,但不同的采供血机构及不同滤器处理的血浆样品间MB残留量仍存在明显差异。
Objective To detect the residual amount of methylene blue (MB) in the plasma of virus inactivation treatment. Methods The plasma samples from four blood collection and supply institutions (A, B, C and D) were treated with two different types of disposable virus inactivation devices, and then the virus-inactivated plasma and standard samples were extracted by solid-phase extraction MB, MB content was detected by high performance liquid chromatography. Results The mean plasma MB inactivation plasma levels of the four blood collection and delivery institutions were (0.29 ± 0.11), (0.26 ± 0.09), (0.09 ± 0.03) and (0.10 ± 0.02) μmol / L, respectively. The filtration rates of MB by two different types of filters were 91% and 73%, respectively, with significant differences (P <0.01). Conclusion At present, MB residues in virus-inactivated plasma in our country basically meet the quality requirements. However, there are still significant differences in MB residues between plasma samples collected from different blood collection and supply institutions and different filters.