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目的研究TNF-α单核苷酸多态性与食管癌发生、发展和转移的关系。方法以PCR-RFL方法分析了食管癌病例(n=202)和按性别、年龄频数对照的正常对照者(n=317)TNF-α-308G/A多态,并比较不同基因型与食管癌发生风险、分化程度以及淋巴结转移的关系。结果TNF-α-308G/A基因频率在病例组与对照组中的分布无显著性差异(p>0.05),与食管癌发生风险无关。携带有A(G/A or A/A)等位基因的食管癌患者,其肿瘤恶性程度显著高于G/G基因型(79.8%&56.1%;OR=2.948,95%CI=1.468～5.920;p<0.05),而且,发生淋巴结转移的风险性显著增加(54.0%&34.5%;OR=2.223,95%CI=1.212～4.076;p<0.05)。结论TNF-α-308G/A多态性可能在食管癌的发展和转移中起一定作用。
Objective To study the relationship between the TNF-α SNP and the occurrence, development and metastasis of esophageal cancer. Methods The polymorphisms of TNF-α-308G / A in esophageal cancer cases (n = 202) and controls (n = 317) by sex and age were analyzed by PCR-RFL. The occurrence of risk, the degree of differentiation and lymph node metastasis. Results There was no significant difference in the distribution of TNF-α-308G / A gene between the case group and the control group (p> 0.05), which was not associated with the risk of esophageal cancer. The malignant degree of esophageal cancer patients carrying A (G / A or A / A) allele was significantly higher than that of G / G genotype (79.8% & 56.1%; OR = 2.948, 95% CI = 5.920; p <0.05). Moreover, the risk of lymph node metastasis was significantly increased (54.0% & 34.5%; OR = 2.223, 95% CI = 1.212-4.076; p <0.05). Conclusion TNF-α-308G / A polymorphism may play a role in the development and metastasis of esophageal cancer.