论文部分内容阅读
目的研究厦门市思明区一起聚集性胃肠炎事件的诺如病毒的分子生物学特征。方法将收集到的11份肛拭子标本及1份生蚝样品,采用实时荧光定量逆转录-聚合酶链反应(RT-PCR)方法检测诺如病毒核酸,阳性标本再进行实时荧光定量RT-PCR扩增,扩增产物经过凝胶电泳分析后,进行序列测定,确定基因型,并进行系统发育树分析。结果 11份肛拭子标本中检出5株GⅠ组诺如病毒株,2株测序成功,检出4株GⅡ组毒株,3株测序成功;1份生蚝样品中检出1株GⅠ组毒株,测序成功。对测序成功的6株毒株进行同源性分析,GⅠ.2型毒株所测序列与2014年上海株KP325648等6株参考株高度同源,GⅡ.17型毒株所测序列与2015年韩国株KT384078等8株参考株高度同源,证实这是一起由GⅠ.2和GⅡ.17型诺如病毒混合感染引起的聚集性胃肠炎事件,GⅡ.17型毒株为厦门市首次报道。结论此次聚集性胃肠炎事件是由诺如病毒引起,且为GⅠ.2与GⅡ.17型毒株混合感染引起。
Objective To study the molecular biology of norovirus in gastroenteritis associated with gastroenteritis in Siming, Xiamen. Methods 11 samples of rectal swabs and 1 sample of oyster were collected. Real-time fluorescent quantitative RT-PCR was used to detect Norovirus nucleic acid and real-time fluorescent quantitative RT-PCR Amplification, amplification products after gel electrophoresis analysis, sequence determination, genotype determination, and phylogenetic tree analysis. Results Five isolates of G Ⅰ coxsackievirus were detected in 11 samples of rectal swabs. Two of them were successfully sequenced. Four strains of G Ⅱ were detected and three of them were sequenced successfully. One strain of GⅠ was detected in one oyster sample Strain, sequencing success. Homology analysis was performed on the 6 strains sequenced successfully. The sequences tested by strain GⅠ.2 were highly homologous to 6 reference strains such as Shanghai strain KP325648 in 2014, and the sequences tested by strain GⅡ.17 were identical to those obtained in 2015 Korea strains KT384078 and other 8 reference strains highly homologous, confirmed that this is a mixed infection with GⅠ.2 and GⅡ.17 norovirus infection caused by gastroenteritis, G Ⅱ 17 strain was first reported in Xiamen City . Conclusions This gathering gastroenteritis event was caused by Norovirus and was caused by mixed infection of GⅠ.2 and GⅡ.17 strains.