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目的：了解大鼠感染性脑水肿脑组织谷氨酸（Ｇｌｕ）与γ－氨基丁酸（ＧＡＢＡ）的变化及黄芩甙对其影响。方法：百日咳菌液诱发大鼠感染性脑水肿及腹腔注射黄芩甙后，用高效液相色谱－荧光检测法测定大鼠脑组织匀浆中Ｇｌｕ和ＧＡＢＡ含量。结果：空白对照组（Ｃ组）、生理盐水组（ＮＳ组）、百日咳菌液组（ＰＢ组）和黄芩甙组（ＢＣ组）大鼠脑组织匀浆Ｇｌｕ含量变化不大（Ｐ＞０．０５），ＰＢ组大鼠脑组织ＧＡＢＡ含量（２．７１±０．２９）增加，ＢＣ组ＧＡＢＡ含量（４．０６±０．５２）增加更明显，与Ｃ组、ＮＳ组、ＰＢ组比较均有显著性差异（Ｐ＜０．０１）。同时ＢＣ组大鼠脑含水量（７９．８±０．４）明显下降，与ＰＢ组大鼠脑含水量（８２．２±０．３）比较有显著性差异（Ｐ＜０．０１）。结论：黄芩甙对大鼠感染性脑水肿有保护作用，其机制可能与黄芩甙能增加感染性脑水肿大鼠脑组织的ＧＡＢＡ含量有关。
OBJECTIVE: To investigate the changes of glutamate (Glu) and gamma-aminobutyric acid (GABA) in brain tissue of rats with infectious brain edema and the effect of Huangqi on it. METHODS: Pertussis bacilli-induced brain edema in rats and intraperitoneal injection of jaundice were used to determine the content of Glu and GABA in rat brain homogenate by high performance liquid chromatography-fluorescence detection. RESULTS: There was no significant difference in Glu content of brain homogenate between control group (C), saline group (NS group), pertussis group (PB group) and Huangqi group (BC group) (P>0. 05) The content of GABA in the brain of PB rats increased (2.71±0.29). The increase of GABA content in the BC group (4.06±0.52) was more obvious than that of the C group, the NS group, and the PB group. There was a significant difference (P<0.01). At the same time, the brain water content (79.8±0.4) in the BC group was significantly decreased, which was significantly different from that in the PB group (82.2±0.3) (P<0.01). Conclusion: Astragalus has a protective effect on experimental cerebral edema in rats, and its mechanism may be related to the increase of GABA content in brain tissue of rats with infectious brain edema.