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研究了温和的碱水解条件对亚麻籽中木脂素(SDG)提取效率的影响。采用响应面法优化并确定了最佳的提取分离条件,并比较了我国不同产地亚麻籽中SDG的含量。研究结果表明,当碱度为3~20 mmol/L时,经足够长的时间水解,均能有效解离SDG聚合物。利用Box-Behnken中心组合设计试验,预测并经验证的最优碱水解条件为:碱浓度13 mmol/L、水解温度53℃、水解时间340.5 min。宁夏和内蒙古产的红色亚麻籽中SDG含量较高,分别为16.29±0.70 mg/g、15.41±0.78 mg/g,河北产的红色亚麻籽中SDG含量为13.13±0.63 mg/g,而河北产的黄色亚麻籽中SDG含量最低,为7.05±0.16 mg/g。研究获得的最优碱水解条件为评估亚麻籽资源品质、高效提取亚麻籽中木脂素及对木脂素进行准确定量等提供了重要的技术支持。
The effects of mild alkaline hydrolysis conditions on the extraction efficiency of lignan (SDG) in flaxseed were studied. The response surface method was used to optimize and determine the optimal conditions for extraction and separation, and the content of SDG in flaxseed from different areas was compared. The results show that when the alkalinity is 3 ~ 20 mmol / L, the SDG polymer can be effectively dissociated by hydrolysis for a long enough time. Based on the Box-Behnken center design, the optimal alkali hydrolysis conditions were as follows: alkali concentration 13 mmol / L, hydrolysis temperature 53 ℃, hydrolysis time 340.5 min. SDG content of red flaxseed in Ningxia and Inner Mongolia were higher, which were 16.29 ± 0.70 mg / g and 15.41 ± 0.78 mg / g, respectively. SDG content of red flaxseed in Hebei was 13.13 ± 0.63 mg / g, Yellow flaxseed had the lowest SDG content of 7.05 ± 0.16 mg / g. The optimal alkali hydrolysis conditions obtained from the study provided important technical support for the assessment of flaxseed resource quality, efficient extraction of lignans from flaxseed and accurate quantification of lignan.