论文部分内容阅读
目的和方法 :采用病例 -对照研究方法和多重PCR技术检测肺癌病例组 16 1人和健康对照组 16 5人的GSTM1(glutathioneS -transferaseM1)和GSTT1(glutathioneS -transferaseT1)基因缺陷型的频率 ,以多因素Logistic回归模型评价GSTM1和GSTT1基因型之间以及基因型与吸烟之间的交互作用。以探讨谷胱甘肽硫转移酶M1和T1的基因多态性与肺癌发病的关系。结果 :GSTM1基因缺陷型和GSTT1基因缺陷型的频率在病例组和对照组之间均无显著的差异。在不吸烟 (SI=0 )的人群中 ,GSTM1基因缺陷型携带者患肺癌的危险性显著增加。此外 ,该基因型还可显著增加年龄≥ 6 0岁者患肺腺癌的危险性。多因素Logistic回归分析显示吸烟和GSTM1基因缺陷型是肺癌的危险因素 ,吸烟与GSTM1和GSTT1基因型不存在交互作用。分层分析表明GSTT1基因功能型与GSTM1基因缺陷型存在明显的交互作用 ,在年龄≥ 6 0岁的人群及不吸烟的人群中 ,GSTT1基因功能型可以使得GSTM1基因缺陷型携带者患肺腺癌的危险度分别降低 48 5 %和 45 3%。结论 :GSTM1基因缺陷型是非吸烟者和年龄≥ 6 0岁者患肺癌 ,尤其是患肺腺癌的危险因素 ,GSTT1基因功能型可以降低不吸烟或年龄≥ 6 0岁的GSTM1基因缺陷型携带者患肺腺癌的危险度。在肺癌的发生过程中GSTM1和GSTT1基因缺陷?
PURPOSE AND METHODS: The frequency of GSTM1 (glutathione S -transferase M1) and GSTT1 (glutathione S -transferase T1) gene deficiency in 16 1 lung cancer patients and 16 5 healthy controls was detected by case-control study and multiplex PCR. Logistic regression models were used to evaluate the interaction between GSTM1 and GSTT1 genotypes and genotypes and smoking. To investigate the relationship between the gene polymorphisms of glutathione S-transferase M1 and T1 and the pathogenesis of lung cancer. RESULTS: There was no significant difference in the frequencies of GSTM1-deficient and GSTT1-deficient patients between the case and control groups. In non-smoking (SI = 0) populations, GSTM1-deficient carriers have a significantly increased risk of developing lung cancer. In addition, the genotype can significantly increase the risk of lung adenocarcinoma in people over 60 years of age. Multivariate logistic regression analysis showed that smoking and GSTM1 gene deficiency were risk factors for lung cancer. There was no interaction between smoking and GSTM1 and GSTT1 genotypes. Hierarchical analysis showed that there was a significant interaction between GSTT1 gene function and GSTM1 gene deficiency in GSTM1 gene-deficient carriers in patients aged 60 years or older and non-smokers The risk of reducing by 48 5% and 45 3% respectively. CONCLUSIONS: GSTM1 gene-deficient patients are risk factors for lung cancer, especially lung adenocarcinoma, in non-smokers and people over 60 years of age. GSTT1 gene function can reduce non-smoking or GSTM1-deficient carriers Risk of lung adenocarcinoma. GSTM1 and GSTT1 gene defects in the development of lung cancer?