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目的研究间充质干细胞的体内免疫调节活性。方法将C57/BL脾脏细胞输注入受亚致死量照射的BALB/c小鼠,建立急性移植物抗宿主病模型,受体小鼠或同时接受密质骨骨髓来源的间充质干细胞,流式细胞技术检测脾细胞表面表达的活性分子。结果模型经输注间充质干细胞后,鼠脾脏CD11b+细胞表达的CD86和CD69减少,提示抗原呈递细胞的成熟度下降。此外,作为一种效应T细胞早期活化和发育的标记,CD3+CD69+细胞/CD3+细胞的比值降低,导致脾内CD3+细胞的绝对和相对数目减少。但是,在同系脾细胞输注模型中,CD11b+细胞表面的CD69和MHCⅡ表达以及CD3+细胞表面的CD69不受影响。结论 MSCs可以分3个阶段抑制急性移植物抗宿主病,有望成为急性移植物抗宿主病的有效治疗手段,但仍需进行深入研究。
Objective To study the immunomodulatory activity of mesenchymal stem cells in vivo. Methods C57 / BL spleen cells were infused into BALB / c mice exposed to sublethal irradiation to establish a model of acute graft-versus-host disease, recipient mice or mesenchymal stem cells derived from cadaveric bone marrow. Cytometric detection of active molecules expressed on the surface of spleen cells. Results After transfection of mesenchymal stem cells, the expression of CD86 and CD69 in murine spleen CD11b + cells decreased, suggesting that the maturation of antigen presenting cells decreased. In addition, as a marker of early activation and development of effector T cells, the ratio of CD3 + CD69 + cells / CD3 + cells decreases resulting in a reduction in the absolute and relative numbers of CD3 + cells in the spleen. However, in the syngeneic splenocyte infusion model, CD69 and MHC II expression on the CD11b + cell surface and CD69 on the CD3 + cell surface were unaffected. Conclusion MSCs can inhibit acute graft-versus-host disease in three stages and is expected to be an effective treatment for acute graft-versus-host disease. However, further study is needed.