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为了探讨中国仓鼠（ＣＨＡ）卵丘细胞及其胞外基质（ＣＯＭ）和细胞外基质（ＥＣＭ）对精子获能、顶体反应（ＡＲ）和受精能力的作用，以及ＣＯＭ中何种因子影响上述受精环节，分别采用阿利新蓝８ＧＸ法和放射免疫法（ＲＩＡ）测定ＣＯＭ中糖胺多糖（ＧＡＧ）总量和孕酮（Ｐ４）含量，并以精子穿卵试验（ＳＰＡ）评价精子的受精能力。结果表明：ＣＨＡ精子在ＣＯＭ中ＡＲ率随获能培养时间的延长而增加，呈时间依赖关系。ＥＣＭ是诱发ＣＨＡ精子ＡＲ的主要部位。它含Ｐ４、透明质酸（ＨＡ）和肝素（ＨＥ）等。它们均可引起ＣＨＡ体外获能精子发生ＡＲ，ＡＲ率随Ｐ４和ＧＡＧ剂量而增加，并存在着明显的量效关系。Ｐ４不能引起未获能精子发生ＡＲ，据此，它可以作为一种评价精子是否获能的探针。此外，Ｐ４还可明显地提高ＣＨＡ精子穿卵能力。由此可得出结论，ＥＣＭ中Ｐ４和ＧＡＧ是引起ＣＨＡ精子获能、ＡＲ和受精的因子
To investigate the effects of Chinese hamster (CHA) cumulus cells and their extracellular matrix (ECM) on sperm capacitation, acrosome reaction (AR) and fertilization ability, as well as which factors in COM affect the above The amount of glycosaminoglycan (GAG) and progesterone (P4) in COM were determined by alginate blue 8GX and radioimmunoassay (RIA), respectively. The fertilization ability of sperm was evaluated by sperm penetration test . The results showed that the AR rate of CHA sperm in COM increased with time prolonging the incubation time in a time-dependent manner. ECM is the main site of CHA induced sperm AR. It contains P4, hyaluronic acid (HA) and heparin (HE) and so on. Both of them can cause CHA in vitro spermatogenesis AR, AR rate increased with the dose of P4 and GAG, and there is a clear dose-effect relationship. P4 can not cause AR in un-enabled spermatozoa, therefore, it can be used as a kind of evaluation of sperm motility is not probable. In addition, P4 can also significantly improve CHA sperm penetration ability. From this it can be concluded that P4 and GAG in ECM are the factors that cause CHA sperm capacitation, AR and fertilization