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目的观察槲皮素(QUE)及异鼠李素(ISOR)对培养人血管平滑肌细胞增殖的影响。方法本实验利用培养的人主动脉平滑肌细胞(VSMC),采用细胞计数、3H-胸腺嘧啶核苷酸(3H-TdR)掺入法,观察QUE、ISOR、去甲肾上腺素(NE)对VSMC增殖、DNA合成的影响,以及QUE、ISOR、酚托拉明(Ph)对NE促VSMC增殖、DNA合成的的影响。结果①QUE有明显抑制VSMC增殖、DNA合成的作用,而ISOR作用较弱。在所观察的1～200(滋mol/L的浓度范围内,QUE和ISOR均在200滋mol/L浓度发挥了最大的抑制作用,其抑制作用有剂量依赖关系。②NE有促进VSMC增殖、DNA合成的作用,而这些促进作用能被Ph所阻滞。③QUE和ISOR均剂量依赖地抑制NE促VSMC增殖和DNA合成的作用,且QUE和ISOR有明显的协同作用。④QUE和ISOR对NE刺激作用的抑制明显强于Ph的作用。⑤QUE和ISOR对VSMC无细胞毒作用。结论QUE和ISOR是细胞毒性很低,对VSMC的增殖、DNA合成尤其是对NE刺激的VSMC增殖、DNA合成有很强的抑制作用的天然黄酮类物质。
Objective To observe the effects of quercetin (QUE) and isorhamnetin (ISOR) on the proliferation of cultured human vascular smooth muscle cells. Methods Cultured human aortic smooth muscle cells (VSMC) were used in this experiment. The proliferation of VSMCs was observed using cell counting and 3H-thymidine nucleotide (3H-TdR) incorporation assays for QUE, ISOR, and norepinephrine (NE). The effects of DNA synthesis, and the effects of QUE, ISOR, and phentolamine (Ph) on NE-promoted VSMC proliferation and DNA synthesis. Results 1QUE significantly inhibited proliferation and DNA synthesis of VSMC, while ISOR had weaker effects. In the range of 1-200 (nil-mol/L) observed, both QUE and ISOR exerted maximal inhibitory effect at a concentration of 200 molmol/L, and the inhibition was dose-dependent. 2NE promotes VSMC proliferation, DNA The effects of synthesis, and these promotion effects can be blocked by Ph.3QUE and ISOR both dose-dependently inhibit the role of NE-promoted VSMC proliferation and DNA synthesis, and QUE and ISOR have a significant synergistic effect.4QUE and ISOR on NE stimulation The inhibition was significantly stronger than that of Ph. 5QUE and ISOR had no cytotoxic effect on VSMC.Conclusion QUE and ISOR have very low cytotoxicity, and they have strong effects on the proliferation of VSMC, DNA synthesis, especially the proliferation of VSMC stimulated by NE, and DNA synthesis. The inhibitory effect of natural flavonoids.