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对Ｋｉｍ等的端粒重复序列ＰＣＲ扩增法加以改进，用ＳＹＢＲＧｒｅｅｎ染色代替ＥＢ染色，建立了细胞端粒酶活性的ＰＣＲ－ＴＲＡＰ定性分析法；同时，用γ－３２Ｐ－ＡＴＰ标记ＴＳ引物，加入内对照ＴＳＫ１，建立了ＰＣＲ－ＴＲＡＰ定量分析法。利用上述方法，对１２株人肿瘤或非肿瘤细胞株进行端粒酶活性分析，结果发现，所有被检细胞株均呈端粒酶阳性，但其活性水平在不同细胞株间有较大差异（２３～６５２ＴＰＧｕｎｉｔｓ）。作者认为，细胞端粒酶活性ＰＣＲ－ＴＲＡＰ分析法，特别是定量分析法的建立，为我们真实准确地了解细胞端粒酶活性水平提供了可能，亦为下一步通过抑制端粒酶活性诱迫肿瘤细胞进入程序性死亡的肿瘤基因治疗研究奠定了基础。
To improve the telomere repeats of Kim et al. By PCR amplification, SYBR Green staining was used instead of EB staining to establish the qualitative PCR-TRAP assay of telomerase activity. At the same time, the TS primers were labeled with γ-32P-ATP, Internal control TSK1, established by PCR-TRAP quantitative analysis. Using the above method, telomerase activity was analyzed on 12 human tumor or non-tumor cell lines and found that all tested cell lines were telomerase positive, but their activity levels were significantly different among different cell lines ( 23 ~ 652TPGunits). The authors believe that the establishment of the telomerase activity PCR-TRAP assay, in particular the quantitative assay, provides us with a real and accurate understanding of the telomerase activity and is also the next step of inducing telomerase activity inhibition Tumor cells into programmed cell death gene therapy research laid the foundation.