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用约氏疟原虫和恶性疟原虫免疫 BALB/c 小鼠,取其脾细胞与 SP2/0细胞融合,获得11株抗恶性疟原虫红内期的单克隆抗体(McAb)。以多种疟原虫(恶性疟,间日疟,卵圆疟,诺氏疟、食蟹猴疟和约氏疟)的感染血片为抗原,进行间接免疫荧光测定(IFA),发现有8株 McAb(M26-32,F5-3F9,F5-4E9)与所试6种疟原虫均发生阳性荧光反应。其中 M26-32除能与中国海南岛的2个恶性疟分离株结合外,与东南亚、非洲、拉丁美洲的6个恶性疟分离株及卢旺达临床病人周围血中的环状体亦呈阳性反应。在与我国不同地区的间日疟反应时,几株 McAb 的 IFA 结果不同,提示不同间日疟原虫株的抗原成分有所差异。这些 McAb 与马媾疫锥虫和弓浆虫均无交叉免疫荧光反应。因此可能用于检测病人血中的微量疟原虫抗原,为早期诊断疟疾提供有力的工具,并可能用于鉴定不同地区的间日疟原虫。恶性疟原虫体外生长抑制试验结果表明,McAb M26-32能部分抑制疟原虫对~3H-亮氨酸的掺入,并能延缓原虫血症的上升,在疟疾保护性免疫中可能起一定作用。
BALB / c mice were immunized with Plasmodium yoelii and Plasmodium falciparum and their spleen cells were fused with SP2 / 0 cells to obtain 11 monoclonal antibodies against Plasmodium falciparum in the erythrocytic stage. Indirect immunofluorescence assay (IFA) was performed with the infected blood samples of various Plasmodium species (Plasmodium falciparum, Plasmodium vivax, Echinus falciparum, Nocardiola malae, Cynomolgus malaria and Plasmodium yoelii) and 8 McAbs (M26-32, F5-3F9, F5-4E9) were positive fluorescence reaction with the tested six kinds of Plasmodium. Among them, M26-32 could not only bind to two falciparum malaria isolates from Hainan Island in China, but also positive to six circumsporozoites from 6 falciparum malaria isolates from Southeast Asia, Africa and Latin America. IFA results of several strains of McAbs differed from each other in the malaria reaction with different regions of China, suggesting that the antigenic components of different P. vivax strains are different. These McAbs were not cross-immunofluorescent with T. equi and T. gondii. Therefore, it may be used for the detection of trace Plasmodium antigens in the blood of patients, providing a powerful tool for the early diagnosis of malaria and may be used to identify Plasmodium vivax in different areas. In vitro growth inhibition test of Plasmodium falciparum showed that McAb M26-32 could partially inhibit the incorporation of ~ 3H-leucine into Plasmodium and delay the increase of parasitemia, which may play a role in protective immunity against malaria.