论文部分内容阅读
青枯病是由细菌引起植物枯萎的维管束病害。除土壤外,带菌的繁殖材料如块茎和苗木也是重要的侵染来源。为了防止病害进一步扩展蔓延,对繁殖材料的检疫是一项重要的工作。 间接血凝抑制技术是一种微量快速的血清学方法,特别适用于检测组织中的病原物(如细菌),无需特殊设备,在2—3小时内即可获得结果。 检测结果证明,用间接血凝抑制技术测定植物青枯病,特异性强,灵敏度高。每毫升含2×10~4个细菌的菌液为明显阳性,每毫升含2×10—2×10~3个细菌的菌液为可疑阳性反应。而且只需制备一种青枯菌菌株的抗血清即可检测各种植物青枯病。 间接血凝抑制技术目前已试用于姜瘟病病姜块的检测和甘薯瘟病的病薯和病苗的检疫。
Bacterial wilt is a vascular bundle disease caused by bacteria that causes plants to wilt. In addition to soil, contaminated propagation material such as tubers and seedlings are also important sources of infection. In order to prevent further spread of diseases, the quarantine of reproductive material is an important task. Indirect hemagglutination inhibition is a rapid, trace-based serological method that is especially useful for detecting pathogenic agents (such as bacteria) in your tissue without the need for special equipment to get results in 2-3 hours. Test results show that indirect hemagglutination inhibition of plant bacterial wilt, specificity and sensitivity. Each ml contains 2 × 10 4 bacilli of bacteria was significantly positive per ml of bacteria containing 2 × 10-2 × 10 3 suspicious positive reaction. And only need to prepare a strain of R. solanacearum antisera can detect a variety of plant bacterial wilt. Indirect blood clotting inhibition technology has now been tested for the detection of ginger blast disease ginger and sweet potato blast disease of potato and quarantine.