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目的分析广东省人民医院不同科室来源的耐甲氧西林金黄色葡萄球菌(MRSA)菌株基因分型同源性,为控制MRSA医院感染流行提供科学依据。方法使用细菌基因组重复序列聚合酶链式反应(Rep-PCR)技术以及高级微生物基因分型系统(DiversiLab)细菌同源性分析技术对50株医院感染的MRSA进行基因分型。结果50株MR-SA分为19个基因型,其中A型20株,为主要流行型别,主要来自于重症监护室(ICU)、呼吸科、康复科、神经外科、心外科等11个病区;B型7株,D型和J型各3株,H型和L型各2株,其余均为1株。结论该院存在着以A型MRSA为主的感染流行,该型菌株可能以克隆株的形式播散。
Objective To analyze the genotyping homology of methicillin-resistant Staphylococcus aureus (MRSA) strains in different departments of Guangdong Provincial People’s Hospital and provide a scientific basis for controlling the prevalence of nosocomial infections in MRSA. Methods 50 hospital-acquired MRSA strains were genotyped using bacterial genome repeat polymerase chain reaction (Rep-PCR) and bacterial homology analysis of DiversiLab. Results The 50 strains of MR-SA were divided into 19 genotypes, of which 20 strains were type A and 20 were the major epidemic types, mainly from 11 intensive care unit (ICU), respiratory department, rehabilitation department, neurosurgery and cardiac surgery There were 7 strains of B type, 3 strains of D type and J type, 2 strains of H type and L type respectively, and the rest were 1 strain. Conclusion There is a prevalence of infection with type A MRSA in this hospital, which may spread in the form of clonal strains.