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[目的]优化拟除虫菊酯类农药多残留检测条件,建立适用于多种菊酯类农药的酶联免疫分析方法。[方法]优化多残留酶联免疫检测方法,建立半抗原对单链抗体的标准抑制曲线。对7种拟除虫菊酯类农药进行交叉反应,计算交叉反应率。[结果]最佳检测条件:包被抗原以质量浓度5 mg/L于37℃包被微孔2 h、单链抗体稀释640倍、稀释液中甲醇体积分数为10%、竞争时间为80 min、HRP/Anti-His酶标二抗稀释质量浓度为1/7000 g/L、显色20 min。该方法对氯菊酯、氯氰菊酯和溴氰菊酯有较强交叉反应,交叉率分别为62.31%、43.43%、36.93%。[结论]成功建立可检测多种拟除虫菊酯类农药的酶联免疫分析方法,为免疫分析试剂盒的研发奠定基础。
[Objective] The research aimed to optimize multi-residues detection conditions of pyrethroid pesticides and establish an enzyme-linked immunosorbent assay (ELISA) suitable for a variety of pyrethroid pesticides. [Method] The multi-residue enzyme-linked immunosorbent assay was optimized and the standard inhibition curve of hapten to single chain antibody was established. Seven kinds of pyrethroid pesticides were cross-reacted to calculate the cross-reaction rate. [Result] The best detection condition: coated antigen was coated with micro-pore at 5 mg / L at 37 ℃ for 2 h, the single-chain antibody was diluted 640-fold, the volume fraction of methanol in the diluted solution was 10%, and the competition time was 80 min , HRP / Anti-His enzyme labeled secondary antibody dilution concentration of 1/7000 g / L, color 20 min. The method of permethrin, cypermethrin and deltamethrin have strong cross-reaction, the cross rates were 62.31%, 43.43%, 36.93%. [Conclusion] The enzyme immunoassay method for detecting a variety of pyrethroid pesticides was successfully established and laid the foundation for the development of immunoassay kit.