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目的:探究Smad7对肝癌细胞增殖和迁移的影响及其临床意义。方法:通过转染pcDNA3.1(+)-Smad7质粒或Smad7的小干扰RNA使得Smad7在肝癌细胞系HepG2和Huh7中过表达或敲减,应用MTT法检测Smad7对肝癌细胞增殖的影响,采用细胞划痕实验以及Transwell实验探讨Smad7对肝癌细胞迁移的影响。采用qRT-PCR检测9例肝癌癌患者手术切除的组织样本中Smad7的表达。结果:过表达Smad7的肝癌细胞增殖能力与对照组相比有明显的下降,而敲减smad7能够促进肝癌细胞的增殖。过表达smad7的肝癌细胞穿过Transwell小室底膜的能力显著下降,而敲减Smad7能够促进这种能力。Smad7在肝癌癌旁组织中的表达显著高于癌组织。结论:Smad7能够在肝细胞肝癌的进展中发挥负向调控作用。
Objective: To investigate the effect of Smad7 on the proliferation and migration of hepatocellular carcinoma cells and its clinical significance. Methods: Smad7 was overexpressed or knocked down in HepG2 and Huh7 cell lines by transfecting pcDNA3.1 (+) - Smad7 plasmid or Smad7 small interfering RNA. MTT assay was used to detect the effect of Smad7 on the proliferation of hepatocellular carcinoma cells. Scratch experiment and Transwell experiments to explore the impact of Smad7 on the migration of hepatocellular carcinoma cells. QRT-PCR was used to detect the expression of Smad7 in the surgically resected tissue samples of 9 patients with hepatocellular carcinoma. Results: The proliferation of hepatocarcinoma cells overexpressing Smad7 was significantly decreased compared with the control group, knockdown smad7 could promote the proliferation of hepatoma cells. The ability of Smad7-overexpressing hepatoma cells to cross the basal lamina of Transwell cells was significantly reduced, whereas Smad7 knockdown promoted this ability. The expression of Smad7 in paracancerous tissues of liver cancer was significantly higher than that in cancer tissues. Conclusion: Smad7 can play a negative regulatory role in the progress of hepatocellular carcinoma.