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用18对SSR引物对96份草莓栽培品种资源进行扩增。在18个SSR位点共获得184个条带,其中多态性条带为172个,多态性条带比率为93.5%,不同引物扩增的多态性条带数为4～19个,平均为9.56个。各位点多态性信息含量(PIC)值在0.5726～0.8885之间,平均0.8066。NTSYS软件进行相似性系数计算,UPGMA法进行聚类分析结果显示,96份来源不同的草莓品种被混杂地聚在一起,草莓品种的亲缘关系与其来源并不存在明显的相关性,中国地方品种的聚类相对集中,中国地方品种与中国选育品种之间亲缘关系相对较远。欧美品种和日本品种的遗传基础要宽于中国选育品种和地方品种。
Nineteen strawberry cultivars were amplified with 18 pairs of SSR primers. A total of 184 bands were obtained at 18 SSR loci, of which 172 were polymorphic and 93.5% were polymorphic bands. The number of polymorphic bands amplified by different primers ranged from 4 to 19, The average is 9.56. The polymorphism information content (PIC) of each loci ranged from 0.5726 to 0.8885 with an average of 0.8066. NTSYS software was used to calculate the similarity coefficient. Cluster analysis by UPGMA showed that 96 strawberry cultivars with different origins were mixed together. The genetic relationship of strawberry cultivars was not significantly correlated with the origin of the cultivars. Clustering is relatively concentrated, and the genetic relationship between Chinese landraces and Chinese breeding varieties is relatively far away. The genetic basis of European and American varieties and Japanese varieties should be wider than that of Chinese breeding varieties and local varieties.