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目的比较肺炎支原体(Mycoplasma pneumonia,MP)RNA实时荧光恒温扩增技术(MP-SAT)与肺炎支原体DNA实时荧光定量PCR(MP-DNA)2种方法对早期诊断肺炎支原体感染的作用以及治疗监测的临床意义。方法采集入院24 h内咽拭子标本,分别用2种方法检测,并对MP-SAT与MP-DNA均阳性的28例患儿进行随访检测。结果SAT法检测RNA的敏感度和特异度分别为84.9%和92.5%,2种方法检测的符合率为89.7%。随着大环内脂类抗生素治疗时间的延长,2种方法的检出率逐步降低。住院治疗第5 d时,MP-SAT和MP-DNA分别降为78.6%和71.4%;但在第10 d和第15 d,MP-DNA的下降幅度明显加快,住院率分别为32.1%和7.1%。结论 SAT技术可简单、快速检测RNA,检测效能和实时荧光定量PCR相当,两者联合检测可显著提高早期MP感染的检出率。MP-SAT转阴时间慢于MP-DNA,部分患儿临床症状消失后仍可检出MP-SAT。
Objective To compare the effect of Mycoplasma pneumonia (MP) RNA Real-Time Fluorescence Thermometry (MP-SAT) and Mycoplasma pneumoniae Mycoplasma pneumoniae real-time quantitative PCR (MP-DNA) on the early diagnosis of Mycoplasma pneumoniae infection and its therapeutic monitoring Clinical significance. Methods Twenty-four hours after admission, throat swab specimens were collected and detected by two methods respectively. Twenty-eight children with positive MP-SAT and MP-DNA were followed up. Results The sensitivity and specificity of SAT method for detecting RNA were 84.9% and 92.5%, respectively. The coincidence rates of the two methods were 89.7%. With the macrolide antibiotic treatment time extended, the detection rate of the two methods and gradually reduced. MP-DNA and MP-DNA decreased to 78.6% and 71.4% respectively on the 5th day after hospitalization; however, on the 10th and 15th days, the decline of MP-DNA was significantly accelerated, with hospitalization rates of 32.1% and 7.1% %. Conclusion SAT can detect RNA in a simple and rapid manner. The detection efficiency is comparable to that of real-time PCR. The combined detection of both can significantly improve the detection rate of early MP infection. MP-SAT was slower than MP-DNA, and MP-SAT was still detected in some children after clinical symptoms disappeared.